Properties of Rhodanese from the Liver of Tilapia Oreochromis Niloticus, in Asejire Lake, Nigeria

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dc.contributor.authorATERE, TOPE GAFAR
dc.contributor.authorAgboola, Femi Kayode
dc.contributor.authorAdemakinwa, Adedeji Nelson
dc.date.accessioned2021-02-16T14:41:21Z
dc.date.available2021-02-16T14:41:21Z
dc.date.issued2014-02-20
dc.descriptionStaff Publicationen_US
dc.description.abstractThe study investigates the purification and characterisation of rhodanese from the liver of the tilapia fish (Oreochromis niloticus) collected from Asejire Lake in Nigeria. This was with a view to understanding the biochemical basis of the survival of the fish in cyanide polluted water. Rhodanese was isolated and purified from liver tissue homogenate of tilapia using CM-Sephadex ion exchange chromatography and Sephadex G-75 gel filtration. The specific activity of the enzyme was 56.86 U/mg. The Km values for KCN and Na2S2O3 as substrates were 0.1240 ± 0.0021 mM and 0.0516 ± 0.0097 mM, respectively. The apparent molecular weight was estimated by gel filtration on a Sephacyl S-400 column to be 35,460 Da. The optimal activity was found at pH 6.5 and the temperature optimum was 40°C. The rhodanese enzyme showed that the activity of the enzyme was not affected by MgCl2, KCl, NH4Cl, MnCl2 and CaCl2 while AlCl3, inhibited the enzyme.en_US
dc.identifier.citationAtere, T., Ademakinwa, A.N., Agboola, F., Gafar, A.T., Kayode, A.F., & Nelson, A.A. (2014). Properties of rhodanese from the liver of tilapia, Oreochromis niloticus, in Asejire Lake, Nigeria. African Journal of Biochemistry Research, 8, 74-83. DOI:10.5897/AJBR2014.0755en_US
dc.identifier.issn1996-0778
dc.identifier.uriDOI:10.5897/AJBR2014.0755
dc.identifier.urihttp://repository.elizadeuniversity.edu.ng/handle/20.500.12398/843
dc.publisherAcademic Journalsen_US
dc.subjectCyanideen_US
dc.subjectDetoxificationen_US
dc.subjectTilapiaen_US
dc.subjectLiveren_US
dc.subjectRhodaneseen_US
dc.titleProperties of Rhodanese from the Liver of Tilapia Oreochromis Niloticus, in Asejire Lake, Nigeriaen_US
dc.typeArticleen_US
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