Development of an Analytical Method for Nitric Oxide Radical Determination in Natural Waters

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dc.contributor.authorOlasehinde, Emmanuel F.
dc.contributor.authorKazuhiko, Takeda
dc.contributor.authorHiroshi, Sakugawa
dc.date.accessioned2019-06-26T12:45:51Z
dc.date.available2019-06-26T12:45:51Z
dc.date.issued2009-07-27
dc.description.abstractThe measurement of photochemically generated nitric oxide radicals (NO) in natural waters has long been an arduous task because of a lack of simple analytical techniques, even though the environmental significance of this radical is paramount. We have developed a simple analytical method for the determination of photochemically generated NO in natural waters using 4,5-diaminofluorescein (DAF-2) as a probe compound. This method is based on the reaction of photoformed NO with DAF-2 in air-saturated solution to produce a highly fluorescent triazolofluorescein (DAF-2T) product. DAF-2T was determined by using reversed-phase HPLC with fluorescence detection, with excitation and emission wavelengths of 495 and 515 nm, respectively. Under optimum conditions, the calibration curve exhibited linearity in the range of 0.025−10 nM DAF-2T. The coefficients of variance for the measurements of the signal intensities of DAF-2T (from the photolysis of 0.5 μM and 5 μM NO2− with DAF-2) were less than 5% and 3%, respectively. For a total irradiation time of 30 min, the detection limit of the photoformation rate of NO was 1.65 × 10−13 M s−1, defined as 3σ of the lowest measured DAF-2T concentration (0.025 nM). The proposed method is relatively unaffected by potential interferents in seawater. The method was employed to determine the photoformation rate of NO in the Seto Inland Sea and the Kurose River in Hiroshima Prefecture, Japan. The measured NO photoformation rates in seawater and river water samples ranged from (5.3−32) × 10−12 M s−1 and (9.4−300) × 10−12 M s−1, respectively.en_US
dc.identifier.citationOlasehinde, E. F., Takeda, K., & Sakugawa, H. (2009). Development of an analytical method for nitric oxide radical determination in natural waters. Analytical chemistry, 81(16), 6843-6850.en_US
dc.identifier.urihttps://doi.org/10.1021/ac901128y
dc.identifier.urihttp://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/173
dc.language.isoenen_US
dc.publisherACS Publicationsen_US
dc.subjectNitric Oxide Radicalen_US
dc.subjectNatural Watersen_US
dc.subjectAnalytical methoden_US
dc.subjectNitric oxideen_US
dc.titleDevelopment of an Analytical Method for Nitric Oxide Radical Determination in Natural Watersen_US
dc.typeArticleen_US
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