Co-metabolic Degradation of Polychlorinated Biphenyls (PCBs) by Axenic Cultures of Ralstonia sp. Strain SA-5 and Pseudomonas sp. Strain SA-6 obtained from Nigerian Contaminated Soils

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dc.contributor.authorAdebusoye, Sunday A.
dc.contributor.authorIlori, Matthew O.
dc.contributor.authorPicardal, Flynn W.
dc.contributor.authorAmund, Olukayode O.
dc.date.accessioned2019-06-20T09:17:27Z
dc.date.available2019-06-20T09:17:27Z
dc.date.issued2008-01-01
dc.description.abstractSubstantial metabolism of 2,3,4,5-tetrachlorobiphenyl (2,3,4,5-tetraCB) and 2,3′,4′,5-tetraCB by axenic cultures of Ralstonia sp. SA-5 and Pseudomonas sp. SA-6 was observed in the presence of biphenyl supplementation, although, the strains were unable to utilize tetrachlorobiphenyls as growth substrate. The former was more amenable to aerobic degradation (∼70% degradation) than the latter (22–45% degradation). Recovery of 2,5-chlorobenzoic acid and chloride from 2,3′,4′,5-tetraCB assay is an indication of initial dioxygenase attack on the 3,4-dichlorophenyl ring. The PCB-degradative ability of both strains was also investigated by GC analysis of individual congeners in Aroclor 1242 (100 ppm) following 12-day incubation with washed benzoate-grown cells. Results revealed two different catabolic properties. Whereas strain SA-6 required biphenyl as inducer of the degradation activity, such induction was not required by strain SA-5. Nearly all the detectable congeners in the mixture were extensively degraded (% reduction in ECD area counts for individual congeners ranged from 50.0 to 100% and 14.2 to 100%, respectively, for SA-5 and SA-6). The two strains exhibited no noticeable specificity for congeners with varying numbers of chlorine substitution and positions. The degradative competence of these isolates most especially SA-5 makes them among the most versatile PCB-metabolizing organisms yet reported.en_US
dc.identifier.citationAdebusoye, et al (2008). Co-metabolic Degradation of Polychlorinated Biphenyls (PCBs) by Axenic Cultures of Ralstonia sp. Strain SA-5 and Pseudomonas sp. Strain SA-6 obtained from Nigerian Contaminated Soils. World J Microbiol Biotechnol 24:61–68en_US
dc.identifier.uri10.1007/s11274-007-9438-z
dc.identifier.urihttp://repository.elizadeuniversity.edu.ng/handle/20.500.12398/66
dc.language.isoenen_US
dc.publisherSpringer Netherlandsen_US
dc.subjectBacteriaen_US
dc.subjectPseudomonas spen_US
dc.subjectRalstonia sp.en_US
dc.subjectBiphenylen_US
dc.subjectChlorobenzoic aciden_US
dc.titleCo-metabolic Degradation of Polychlorinated Biphenyls (PCBs) by Axenic Cultures of Ralstonia sp. Strain SA-5 and Pseudomonas sp. Strain SA-6 obtained from Nigerian Contaminated Soilsen_US
dc.typeArticleen_US
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