Browsing by Author "Isanbor, Chukwuemeka"
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Item The degradation of coniferyl alcohol and the complementary production of chlorogenic acids in the growth culture of Streptomyces albogriseolus KF977548 isolated from decaying wood residues(Elsevier, 2017-01-01) Buraimoh, Olanike M.; Ilori, Matthew O.; Amund, Olukayode O.; Isanbor, Chukwuemeka; Michel Jr, Frederick C.Coniferyl alcohol is one of the major precursors of lignin; the most abundant aromatic compound and a natural resource currently receiving attention because of the value-added metabolites resulting from its degradation. Growth study of Streptomyces albogriseolus KF977548 (strain AOB) isolated from decaying wood residues in a tropical estuarine ecosystem was carried out using coniferyl alcohol as a sole carbon source. Cell growth and metabolite production were monitored at 24 h interval by dry weight measurements and HPLC, LC–MS-DAD analyses. Biochemical and PCR assays were carried out to detect the major catabolic enzymes of interest. Strain AOB utilized coniferyl alcohol completely within 72 h ( = 0.204 h−1, Td = 3.4 h). Laccase and peroxidase were released into the growth medium up to 0.099 and 98 mol/mL respectively. Protocatechuate 3, 4-dioxygenase and demethylase were detected in the genome whilst ortho-adipate pathway was clearly indicated. Growth on coniferyl alcohol or caffeic acid as mono substrates resulted in the production of secondary metabolites identified by HPLC–MS as 1- caffeoylquinic and 3,4,5-tricaffeoylquinic acids, known as chlorogenic acids, in the culture medium. The microbial production of chlorogenic acids from a lignin-related substrate base by strain AOB could arouse a plausible biotechnological process.Item Sustainable generation of bioethanol from sugarcane wastes by Streptomyces coelicolor strain COB KF977550 isolated from a tropical estuary(Scientific African, 2021-01-20) Buraimoh, Olanike Maria;; Ogunyemi, Adewale Kayode; Isanbor, Chukwuemeka; Aina, Oluwafemi Segun; Amund, Olukayode Oladipo; Ilori, Mathew Olusoji; Familoni, Oluwole BabafemiThe damaging effect and challenges associated with the use of fossil fuel is enormous and very costly. Biofuels could be obtained from plant biomass wastes which are known to be sources of environmental pollution and breeding grounds for vectors of diseases. Sugarcane bagasse was exploited as a renewable substrate for obtaining bioethanol using Streptomyces strain COB KF977550 as inoculum. Submerged aerobic batch fermentation was performed in flasks containing mineral salts medium supplemented with 5.0 g (w/v) sugarcane bagasse. Incubation was done in a shaker (150 rpm) at 30 oC for 21 days. Microbial growth was assessed by measurement of the optical density (O.D 600nm) at 3-day intervals. Fractional distillation was carried out in batch mode using a simple fractional distillation setup. Metabolic products were determined using GC-FID. Further analyses were performed using FTIR and GC-MS. The optical density of S.coelicolor strain COB KF977550 increased from 0.9 to 1.41. The GC-FID showed that 43.08 g/L ethanol was generated. Interestingly, the results showed the presence of diverse biochemicals released into the medium in addition to the main product ethanol. Ten carboxylic acids including formic acid, glycolic acid, tartaric acid, acetic acid, citric acid, oxalic acid, malic acid, lactic acid, n-valeric acid, and 3-hydroxybutyric acid were identified as biochemical organic acids by-products.